Dihydroartemisinin ameliorates palmitate-induced apoptosis in cardiomyocytes via regulation on miR-133b/Sirt1 axis

Excessive fat ectopically deposited in the non-adipose tissues is considered as one of leading causes myopathy. The aim this study was to investigate role Dihydroartemisinin (DHA) palmitate (PAL)-incubated H9c2 cells (lipotoxicity-induced cell injury model). Cell viability PAL-treated determined by MTT assay, and apoptotic regulators were examined qRT-PCR western blot analysis, absence or presence DHA, respectively. Expression levels miR-133b Sirt1 also evaluated blotting examination. PAL decreased enhanced expression genes. DHA reversed effect on lowed level Caspase3 Bax. It lowered miR-133b, while Bcl-2. revealed target through transcriptional regulation process affected DHA. partially protected against PAL-induced lipotoxicity influencing that hindered activity Sirt1. may be used a potential treatment clinical management for induced heart complications.